Journal: Oncotarget

Article Title: Upregulated TRIM29 promotes proliferation and metastasis of nasopharyngeal carcinoma via PTEN/AKT/mTOR signal pathway

doi: 10.18632/oncotarget.7215

Figure Lengend Snippet: A. Western blotting reveals a reduced expression of epithelial makers (E-cadherin and α-catenin) and an increased expression of mesenchymal markers (fibronectin and vimentin) in TRIM29-expressing S-18 and 6-10B cells in comparison with the vector controls. GAPDH was used as a loading control. B. Western blotting assay shows increased levels of E-cadherin and α-catenin, and decreased levels of fibronectin and vimentin in 5-8F-shTRIM29 cells compared with that in shNC treated cells. C. Over-expression of TRIM29 promoted S-18 cell invasion and metastasis in athymic nude mice in vivo . Left, representative images show lungs with metastatic NPC tumors; left (lower), number of metastatic nodules formed in the lung of mice 6 weeks after tail vein injection of S-18-vector and S-18-TRIM29 cells (5 mice per group; p < 0.01; independent Student's t- test); right, Examples of haematoxylin and eosin staining in two lung samples originating from S-18-vector and S-18-TRIM29 cell-injected mice (magnification 400×).

Article Snippet: Rabbit polyclonal antibody against human TRIM29 was purchased from Abcam; mouse monoclonal antibodies against human α-catenin, E-cadherin, fibronectin and vimentin were purchased from BD Biosciences; rabbit monoclonal antibodies against human phospho-AKT (Ser473), AKT (pan), phospho-p70S6K (Thr389), p70S6K, phospho-4E-BP1, 4E-BP1 and PTEN were obtained from Cell Signaling Technology.

Techniques: Western Blot, Expressing, Plasmid Preparation, Over Expression, In Vivo, Injection, Staining